What chances do I have to re-infect cells from a purified form of viral RNA (+sense), if the cells where treated with any sort of poration or rupturing for cell membrane, do you think the viral RNA can invade cells and replicate?
https://www.ecdc.europa.eu/sites/default/files/documents/covid-19-supply-substances-human-origin.pdf
There are evidences that viral RNA was present in stool samples of patients whose respiratory tract was cleared from the virus. But there were no evidences about viral transmission through stool/urine. That means though viral RNA was there it cannot infect and produce the virus again.Therefore, I do not think it is possible.
Most likely not because infection requires spike protein.
Try for detection an old SARSCoV antibody:
Article A SARS-like Coronavirus was Expected, but nothing was done t...
Without proper envelope and other surface accessories, SARS-CoV-2 RNA may still enter the cells in the in vitro setting. This has been shown to occur in some experiments using pure dsRNA to induce RNAi in the cell culture. However, whether this is true in the in vivo situation, we do not know yet. I personally think it would be difficult for the virus RNA (without envelope and others) to enter the cells without triggering robust innate immunity.
Ameer S. Alfatlawi No, I don't think so, because it needs the spike protein on its membrane to interact with the ACE2 receptor on human cells. The interaction also needs enzymatic reaction. However, what about if you use a micro-needle to deliver these naked virus RNA into a living cell? Can this virus RNA still make its own capsid, membrane plus membrane proteins and replicate itself??
I think you can stimulate COVID-19 production by harvesting the virus extract not the RNA in cell cultures then stimulate the virus assembly and production but the main problem will be in identifying the design of the experiment itself. I suggest reading this article about the stimulation of HIV virus Article Sensitivity to electrical stimulation of human immunodeficie...