Does PFA transcardial perfusion artefactually stretch the rodent heart?

Hi everyone I'm comparing aorta/ventricle wall thickness of mouse. Perfusion was done to minimise auto fluorescence during confocal IF. Question is whether transcardial perfusion artefactually...

28 October 2020 8,059 2 View

How do you check your Generalized Linear Mixed Models?

Forgive the lack of a reproducible example in this question, as my problem stems from analysing a large (>50000 rows) dataset. I want to ask a question about generalised linear mixed effects...

13 February 2020 5,437 4 View

Is segmented beta regression possible in R?

Hi, I'm trying to apply segmented regression to a response variable that is bounded by 0 and 1, however I am struggling to interpret the error I have encountered. Reproducible example in R: data1

27 November 2019 3,133 12 View

Mixing Stakeholders in Focus Groups, getting genuine feedback?

Hi all, Have to run some focus groups across a very diverse (government, industry and academia) and sometimes contentious collaborative project. The objective is to find out what each different...

19 August 2019 8,544 8 View

Action Research Project in Management: How long??

Hello all, I am undertaking an action research project with a thesis as an output. Domain and topic are defined and I have been collecting data points and observations for a number of years, in...

16 January 2019 153 4 View

Pressure cooker Antigen retrieval, Same Heating profiles for ALL buffers?

26 October 2016 2,065 2 View

How do I optimize IHC antibody?

Gene discovery usually leads to knock down studies etc and one will usually end up requiring to test and optimise the antibody for the protein in question. Currently, I have western blots to test...

20 December 2015 529 3 View

Is it possible that methanol causes degradation of compounds over time in methanolic extract?

I have a methanolic extract of a type of plant, which contains compounds with bioactivity. However, I noticed that while fresh extracts produces 4 bands of bioactive compounds on a TLC, older...

15 August 2015 1,602 10 View

Are there any research comparing catfish and sharks for any reason?

I have been studying the numerous similiarities that the catfish and sharks have in common. I have studied the writings of Dr. D. Whitehead of Australia as he compared the Ampullae of Lorenzini...

30 April 2014 8,138 7 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

How to get better phase shift in C-V curves for ferroelectric devices?

I tried to sweep my ferroelectric devices from -2V to 2V and then from 2V to -2V and The two hysteresis peaks are off by several microFarads. Should i increase bias voltage? I am doing these...

16 February 2021 8,514 2 View

What is/are the best ML classifier(s) for Sentinel2 images?

Hi I am trying to segment a sentinel2 image. At this stage, I want to run a binary classifier that assigns each pixel to either farm or non-farm pixel. For this purpose, I have 4 10m bands...

15 February 2021 3,596 9 View