I'd like to do a bioinformatic comparison of the observed variable and constant regions of each identified nanobody and/or single chain antibody for the purposes of in vitro synthesis. I'm having a hard time finding explicit cDNA library sequences. Are these deposited anywhere?
Additionally, has anyone done the above already? Perhaps I have overlooked the manuscript.
EDIT:
Found the following database...
http://www.imgt.org/
Has some of the information I'm trying to hunt down. Still interested in whether the actual lymphocyte cDNA libraries have been sequenced and deposited.
http://ican.ils.seu.edu.cn/
iCAN (Institute Collection & Analysis of Nanobody) has been created to expand and accelerate nanobody studies. Nanobody has family-specific sequence composition which can be mined to discover and design novel nanobody sequence.
iCAN contains information on the collected 2391 unique nanobodies. The collection of the database covers all publically available nanobodies that we could obtain from all sources including academic publications and industry patents as well as that generated in our lab. To our knowledge, it is the first public database of nanobody. Information related to sequence, protein definition, accession number, activity, target antigen, patent description and link to databases like PDB, PubMed and other related databases are provided for the benefit of users. The database not only collects the nanobody information, but also provides tools for sequence alignment and key words-based search.
Comprehensive documentation of the database can be obtained from the Help page.
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