I need to determine the glycogen in both liver and cardiac muscle, so I need another method to do different from the Anthrone reagent.
For glycogen, you can look at:
Keppler, D., Decker, K., 1984. Gylcogen determination with aminoglucosidase. In: Bergmeyer, J., Marianne, GraBl (Eds.), Methods of Enzymatic Analysis, “Metabolites I: Carbohydrates”, vol. VI. Verlag Chemie, Florida, p. 701.
and for glucose:
Kunst, A., Draeger, B., Ziegenhorn, J., 1984. D-glucose. UV-method with hexokinase and glucose-6-phosphate dehydrogenase. In: Bergmeyer, J., Marianne, GraBl (Eds.), Methods of Enzymatic Analysis, “Metabolites I: Carbohydrates”. Basel, vol. VI. Verlag Chemie, Florida, p. 701.
Both methods were applied recently in:
Torre, L., Abele, D., Lagger, C., Momo, F., & Sahade, R. (2014). When shape matters: strategies of different Antarctic ascidians morphotypes to deal with sedimentation. Marine Environmental Research, 99, 179–187. doi:10.1016/j.marenvres.2014.05.014
We use the amyloglucosidase method, which is very reliable for muscle and liver tissue samples and widely published (from Chan and Exton, 1976, originally). Protocol is attached.
We had used successfully the amyloglucosidase method in measuring glycogen content in frog liver and sceletical muscle.
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