Is there a good protocol to generate anti-idiotypic murine antibodies?

10 October 2014 1 3K Report

We are interested in examining the effects of anti-idiotypic antibodies in the alloimmune response. For antibodies generated between mouse strains, is there a good way to generate anti-idiotypic antibodies and purify them?

Lars Hangartner

Hi Steven,

We torelerized rats against murine Ig and immunized the afterwards with the mAbs. The Immunization protocol should be outlined in the attached publication.

Best

Lars

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