How to report a post-hoc comparison for robust mixed ANOVA?

Hi all I have conducted a robust mixed design ANOVA using WRS2 in R. My data are time taken in seconds, and not normally distributed. I cannot remove outliers as the experiment is based around...

23 July 2020 6,766 4 View

One possible process for implementing environmental sustainability?

Can the process be named and list 3 phases of how it would be implemented

14 July 2020 6,249 11 View

How do I produce a collimated image "beam" in my optical system?

I am trying to design an optical system to produce stereo images of an object with a single camera. To do this, I am going to utilize three mirrors: a reflector at each stereo angle and a...

13 July 2020 1,532 4 View

Can someone translate this paragraph into plain English?

Hi all, I'm looking into observational studies to try and better characterize treatment-resistant depression. I'm having trouble deciphering the paragraph in bold below, having read it multiple...

19 February 2020 9,823 2 View

What can you tell me about an E.coli strain named Lemo21 from NEB?

I am currently trying to express human tyrosinase in E.coli and as you know this enzyme being a membrane protein it comes with a fair share of difficulties relating to expression in BL21(DE3)....

03 October 2018 6,996 3 View

Does anyone know of a working antibody against Orexin-A/Hypocretin-1 for immunofluorescence in Brain & Spinal Cord?

Our working antibody (https://www.scbt.com/scbt/product/orexin-a-antibody-c-19) has been discontinued, and we are looking for a replacement. We have tried 3 other antibodies (see below) to...

14 January 2018 9,040 5 View

Protein purification protocol?

Hello fellow scientists, here i have a bit of a seemingly demoralizing issue with protein purification. Could you please help? I have attached a commassie image for clarity. 10mM imidazole, 50 mM...

25 December 2017 3,853 4 View

Can anyone recommend a good recrystallization solvent for THCA that does not dissolve at room temperature, but does when heated?

We have currently been able to isolate THCA (Tetrahydrocannabinolic acid) to upwards of 99% purity with lab tests to confirm potency. We have only had success recrystallizing this compound to...

15 May 2017 6,795 1 View

Is it possible to rescue tissue from paraffin embedding for dissection and RNA extraction?

I would like to know whether it is possible to rescue tissue from paraffin embedding, so that it could be dissected and used for RNA extraction. If so, could somebody recommend a protocol for...

14 July 2016 8,328 4 View

Effective methods of measuring the thickness of hydrophobic nanolayers?

I am working for a nanotechnology company in which we coat various substrates with a hydrophobic nanolayer in order to make the surfaces water-resistant. We are looking for ways to measure the...

25 May 2016 7,320 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

How to find if Qualitative and Quantitative (discrete) variables have a relation?

I'm going to do a brief research about the dependance between the number of cases of Covid-19 and sex( male/female) . One of the requierements is to use inferential statistics to test the...

18 February 2021 7,092 11 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View

Is there any publication about the effect of basketball and volleyball on body height?

Many people believe that some stretching sports with vertical jump such as basketball and volleyball can increase the body height. On the other hand, others do not agree with this argument. The...

02 February 2021 281 6 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How would you assess protein binding adjusted EC50 for a drug that has a binding rate of over 95%?

I'm in need of a reference or a method to come up with a protocol that allows one to get the protein-adjusted EC50 for an antiviral drug in presence of human serum albumin and acid glycoprotein.

27 January 2021 1,402 1 View

Where can I find the receptor binding domain (amino acid sequence) of ALL pathogenic viruses?

I want to compare the RBD of all pathogenic viruses? Is there a database or manuscript that anyone knows of where I can access these?

25 January 2021 3,843 4 View

Can anyone helps me with Autodock 4.2 scripts error ?

After opening the file, I operate command "Script" --> "Ligand Interactions" --> "Show Ligand Binding Site Atoms" and the warning appears as "This script displays ligands and surrounding...

15 January 2021 5,360 1 View