Please tell me, is there a difference between testing a four-port device directly with a four-port VNA and testing a four-port device with a two-port VNA with the other two unexcited ports connected to matched loads?

When I tested the differential line, I used a two-port VNA because I didn't have a four-port VNA, but the reflection coefficient of the test result was much worse than the simulation result, going from below -15dB in the simulation to only staying below -10dB. In my simulation, however, I added the SMAs at exactly the same dimensions as in reality, and the simulation showed that the reflection coefficient would not be as bad as the test results.

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