Is there a difference between the method of extraction for PCR and Spoligotyping?

12 February 2019 0 7K Report

I used the same DNA that I use for spolygotyping to the MIRU-VNTR genotyping for PCR but after amplification the agarose gel revealed nothing except the ladder.

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How the circular economy helps protect the environment?

Good evening colleagues, I'm looking for a thesis proposal on this topic.

24 June 2024 7,782 2 View

I would like to make article screening with RevMan? Any idea?

I am conducting a systematic and want to use RevMan to perform the review. However, I am blocked wit the screening . When I click on studies awaiting classification, I don't see the abastract and...

09 December 2023 329 2 View

Good evening colleagues. I am a teacher-researcher in Mali. I would like to do a research project on the theme of sustainable development?

Good evening colleagues. I am a teacher-researcher in Mali. I would like to do a research project on the theme of sustainable development. To do this, I would like your advice on the choice of theme.

13 September 2023 6,930 2 View

Do TEEs (Trusted Execution Environments) efficiently avoid Byzantine faults?

Can TEEs (Trusted Execution Environments) based software be entirely trusted and capable of preventing malicious blockchain nodes from misbehaving? Or it is a premature assumption because of...

26 November 2022 7,612 4 View

Tasseled cap with landsat for arcgis .tbx extension tools?

Dears members, I am working on shoreline delineation using landsat 8 OLI, Could you please provide me a link to download the tasseled cap with landsat for arcgis .tbx extension for arcgis...

09 May 2022 2,896 2 View

Dear colleagues, what statistical software (packages) do you use to work with complex (household) survey data?

If you use different tools for different steps (sampling selection, weighting, estimation, etc.), please specify the breakdown.

19 May 2020 9,001 3 View

CNN outperformed LSTM in Speech emotion recognition?

Hello I work with Convolutional Neural Network and LSTM in speech emotion recognition, in my result I see that CNN has shown better performance than the traditional LSTM in my speech...

04 September 2019 3,057 11 View

How do you combine multiple SEQ files iinto one file (FASTA or notepad)?

I use MEGA X for my phylogenetic analysis and sometimes I may work with 70+ sequences. If any of you know a method that can combine these sequences into one and free me from the ////tedious////...

31 July 2019 3,307 3 View

Which are best features for emotion recognition?

Hello everyone I'm working in audio analysis for emotion classification. I'm using parselmouth (a PRAAT integration in python) to get feature. I'm not well versed in audio analysis, starter. After...

18 June 2019 8,128 4 View

Hello i would like done a medical image segmentation by neural networks with matlab can you give me indications?

Especially the SOM.

29 August 2018 6,747 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View