Hello. F0 value may depend on the Chl concentration and differ amongst different species, as well as in a particular plant in relation to the stage of growth. However, some alterations occur as a response to the conditions which the plant is subjected to. In this case, some alterations occur in the PSII such as inhibition of its acceptor side, QA reduction, and dissociation of LHCII from PSII. So the explanation is not easy :).
Thanks a lot for this guidance. I am growing two types of plants under low light stress. One is japonica variety Akitokomachi and its Phytochrome A mutant, A0410. I am seeing the total chlorophyll content of A0410 is increasing than Akitokomachi under shade( having 25% natural light). Can you please let me know the expected result for Fv/Fm for Akitokomachi ( wild type) and its Phytochrome A mutant ( A0410).
Please guide me :)
Hi Darshan. Some papers no finding relationship between chlorophyll content and fluorescence. Making a link with Renata said, may occur a inhibition in the acceptor side (ex: application of DCMU), causing modifications in the chlorophyll fluorescence without chlorophyll content degradation.
Now, Fv/Fm is a quantification of maximum quantic yield of the primary photochemistry (TRo/ABS). This parameter is dependent of the numbers of the active reaction centers in the PSII. It's possible that you find Fv/Fm about 0,75 to 0,8 (expected for healthy plants).
Not hesite in ask if you have more questions.
(obs: I'm sorry for my bad english!)
Hi Darshan, so far there is no conclusive evidence on your question, however, some researchers have ariesgado to make correlations on these variables, perhaps, the biggest limitation is the methodological concept to evaluate it, possibly the technique of fluorescence imaging can help in this area, therefore, you can locate the area of interest and this make the respective correlations. On the other hand, the limitation may be that are talking to variables in most cases is derived from mathematical calculations, due to a biophysical analysis, however it is a door that abriri should be to try to explain this correlation. successes and good work.
I was talking about the work of the Toth and your co-works when I said that have no found change in the chlorophyll fluorescence in relation the content!
Excuse-me the exageration, but is excitant and very cool to see she to answer this question!
There is a linear correlation between Chla concentration and the Fo value only for very diluted algae samples. In more dense conditions there is reabsorption of the fluorescence radiation and shading of a part of the sample. Especially in leaves there is only a rough estimation possible since the leaf optics also play a big role.
You can refer to the following publication to observe the regression line between MODIS Chl-a and nFLH:
Conference Paper Investigation the Behavior of MODIS Ocean Color Products und...
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