I would like to inquire about whether the method of squeezing seeds by means of a machine to extract its oil is feasible to study in the MTT experiment in tissue culture lab ?
This is feasible. You will need to define and construct your experimental design first, and the particular seed sample you are interested to work with. A mechanical oil presser machine will do to extract the fixed oil. And then, you will need to determine what kind of cell line you want to test it. Probably you would want to investigate its medicinal property thru MTT assay.