Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,090 3 View

Recommendations for IL-10 Receptor staining?

11 October 2020 9,412 3 View

What is appropriate to use as my affiliation for a manuscript after finishing graduate school?

I would like to publish my graduate (M.S.) research, which is wildlife/conservation-related, and which I conducted at a U.S. university. It's been a few years since I finished graduate school. I...

14 September 2020 6,153 19 View

Do you mind answering a quick survey ? One minute only ?

Hi How are you ? languages are important in our world.. Mostly for you, if you need us to proofreading your articles. You need good translators to do so.. I set up my translation agency by the...

23 May 2020 1,323 3 View

What marker can I use to analyze dendritic spine maturity on a tissue section?

I would like to quantify the number of dendritic spines in all the maturation stages by using a coronal section of a mouse brain. I will be looking more specifically in the hippocampus.

30 September 2019 7,816 5 View

How do I write a PDB file with custom non-atom particles for a coarse grain model for molecular dynamics simulations (on GROMACS)?

I am trying to write custom particles to a PDB file for coarse grain molecular dynamic simulations in GROMACS. In particular, I am trying to model RNA nucleotides using a handful of beads per...

05 July 2018 7,785 2 View

Uninvited guest in intestinal organoids?

Please excuse the poor photograph. This very large body has appeared in my mouse intestinal organoid culture. To the right (out of focus) is my budding organoid, and in the centre is this very...

14 February 2017 6,791 2 View

What causes huge black dots on (nitrocellulose) membranes?

Dear all, I transferred proteins labeled with a fluorescent dye from (a part of) a 2D SDS-PAGE gel to a nitrocellulose membrane. After scanning the nitrocellulose membrane to check whether the...

27 October 2016 2,816 3 View

What is the best method to avoid contamination between samples that need to be homogenized prior to DNA extraction?

We have many samples to be homogenized and the material need to be washed between samples to avoid DNA contamination between them. It is hard to effectively bleach all the materials. I did some...

01 May 2016 443 6 View

How can we assess emotional attachment?

Preparing a presentation on the Theory of attachment and would like to have my fellow classmates do an exercice or answer a questionnaire on the subject. Any ideas of the type of questions I could...

29 February 2016 4,022 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View