How to calculate stokes shift of a molecule which is having absorbance at 307 nm and emission at 469 nm?

how to calculate stokes shift of a molecule which is having absorbance at 307 nm and emission at 469 nm?

10 November 2016 4,673 8 View

What is the effect of donating groups and with drawing groups on fluroscence of imidazo pyridine derivative connected to a moiety having ESIPT ?

what is the effect of donating groups and with drawing groups on fluroscence of imidazo pyridine derivative connected to a moiety having ESIPT ?

10 November 2016 4,974 0 View

What is the effect of isocyanide susbtituent on imidazo [1,2 a] pyridines fluorescence in GBB reaction?

what is the effect of isocyanide susbtituent on imidazo [1,2 a] pyridines fluorescence in GBB reaction? example ; t-butyl, cyclohexyl, 2,6 di-me phenyl, 4-OMePh, benzyl isocyanides

10 November 2016 5,780 0 View

Is push and pull mechanism related to Imidazo(1,2,a) pyridine-3-amines synthesized by Grobke-blackburn beinyme reaction?

Is push and pull mechanism related to Imidazo(1,2,a) pyridine-3-amines synthesized by Grobke-blackburn beinyme reaction

05 June 2016 7,259 1 View

How to synthesize 1-(4-formylphenyl)piperidine-4-carboxylic acid

can any body suggest how to synthesize 1-(4-formylphenyl)piperidine-4-carboxylic acid)

03 April 2016 8,983 0 View

Is 1-(2-bromophenyl)-2-naphthaldehyde fluorescent and it comes polar to 1 bromo-2-napthaldehyde in TLC?

is 1-(2-bromophenyl)-2-naphthaldehyde fluorescent and it comes polar to 1 bromo-2-napthaldehyde in TLC?

08 September 2015 7,113 1 View

Why C-3 position is more reactive than C-2 in imidazo pyridines?

04 May 2015 4,240 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View