How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Does anyone know why I would have unexplained emission overlap for flow cytometry?

Hello, I am new to flow cytometry, and was just using a sample cell population to train with the technician. The cells were PAC-1 cells split into four groups. Unstained, transiently transfected...

28 January 2021 969 2 View

Does anyone know of a library for R that includes phi?

I'm looking to produce some phi correlations and I'd rather not do it manually, but I can't find a library for R that includes phi.

18 January 2021 2,808 9 View

In SEM, how to interpret standardised regression weights and their significance?

I have a SEM model that I'm having difficulty interpreting. It has one direct effect with a Beta/standardised regression weight that appears to be high (0.80) and significant (p

31 December 2020 7,604 6 View

Does anyone know how to add custom text to a violin plot in r, ggpubr made using the ggviolin line?

I've produced a violin plot for a point-biserial correlation. Main title is there, x and y are labelled, but I want to add a custom text box with the correlation coefficient and significance...

26 December 2020 4,480 4 View

How do I interpret these correlations and p-values?

I've just run a series of point biserial correlation tests in R between whether or not characters were assigned national identities, and attributions given to their behaviours - results shown in...

17 December 2020 9,825 4 View

Is it possible to fuse Deep Learning Net and Clustering Algotrithm?

What I did (unpublished yet) is extract several (e.g. 16) mask features within Mask R-CNN from multiple images, construct a distance metric to compare them to predict the image class. Each set of...

13 December 2020 9,504 2 View

Are there models trained+evaluated on multiple datasets?

Generalization of COVID-19 models is a very serious challenge. I'm trying to find a model (ct scans or x-rays) that was trained and evaluated on multiple datasets. It would be particular...

03 December 2020 8,863 4 View

Are there models trained on both x-ray and ct scans for COVID-19 prediction?

I've only come across models that were trained and evaluated on one of these data. I don't see why it would be a problem to do both, as there are many similar features.

03 December 2020 8,310 3 View

When examining a series with different numbers of episodes per series, should I treat it as single sample, use harmonic mean or some other approach?

I'm currently examining how the behaviours of fictional characters are explained to child audiences through attribution theory as part of a study into the psychology of how children learn about...

27 November 2020 8,229 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

Is methylene blue staining necessary for Macroscopic evaluation of the incidence of polyps in a DMH induced colon cancer in mice?

Am working on a DMH induced colon cancer in mice and i would like to know, could polyps or ACFs be counted through visual macroscopic examination without methylene blue staining ? Thank you.

01 February 2021 4,237 2 View

No signal/bands observed on western blot for ERK signaling?

My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...

30 January 2021 1,039 3 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Will rat colon cancer cells (DHD/K12) derived from BDIX rats grow in Sprague Dawley ?

we plan to induce colon cancer by injection. Cell lines derive from BDIX rats, but our experimental setting would favour SD rats. any experience?

23 January 2021 1,437 2 View