Is possible to make an effective Hif-reporter plasmid in the lab or is always necessary by it from a company? Is possible using digestions and ligations make a reporter plasmid to mesure HIF or other transcriptional activity?
Martin Devonshire
We routinely make transgenic lines in xenopus utilizing fluorescent fusion proteins to measure gene expression, the gateway cloning system is a simple way of generating these vectors for transgenesis
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