How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Has somebody seen a strange pattern when transiently expressing Prot-GFP fusion in Benthamiana leaves?

Hi, Has somebody seen this strange aggregation before? I don't think they are agrobacteria since they are GFP specific (only excitable by 488 nm laser). Any input is welcomed. Thanks Alaeddine

29 October 2020 2,641 3 View

How can I calculate sample size for a cross sectional comparative study?

I'm doing a study on comparing the prevalence and severity of extrapyramidal side effects between patients with schizophrenia who smoke tobacco and who don't. How can I determine the sample sizes...

15 September 2020 4,322 3 View

Why is my protein a smear when I detect with CoIP lysis buffer plus LDS?

Hi I am trying to express my protein R in tobacco leaves and do a CoIP, but I can't detect the protein R in CoIP input. I took the same tissue and added 8M Urea plus LDS protein buffer, protein R...

31 August 2020 9,534 10 View

Which component of MPOWER toolkit has shown the highest impact in India since GATS -1?

We know there is a substantial reduction in tobacco use in India as per GATS-2. Which component of MPOWER toolkit has shown the highest impact in India since GATS -1?

25 August 2020 9,254 1 View

How do Trichogramma parasitoids find their hosts?

I am planing an experiment to investigate the dispersion ability of Trichogramma dendrolimi under semi-field conditions. As far as I know Trichogramma uses kairomones for host finding but are...

29 June 2020 4,262 1 View

Need for an appropriate journal for these keywords: Asthma, Environmental tobacco smoke, behavioral interventions?

If you know a good journal about the keywords mentioned above and the following features, please let me know. regards 1) isi - scopus or pubmed indexed 2) low impact factor 3) high acceptance...

14 May 2020 7,742 6 View

What is the best methodology to do this study?

I'm writing about a type of smokeless tobacco used in asia (nicotiana rustica linn), my purpose is to find out about heavy metals such Cd, Ni, Zn, Cu and etc in nicotiana rustica linn.

02 March 2020 4,180 2 View

Is there a way to improve predation on insects (black aphids)?

How to increase the nutritional preference of an insect Coccinella Spetempunctata (L ) To increase catching Aphis fabae.

16 January 2020 8,071 2 View

In my research study, participants were asked about the attitude of tobacco use by using 5 point Likert scale. How can I interpret the findings ?

I have 378 participants and have 18 questions regarding 5 point Likert scale. The questions are like this I believe that most of the friends in my age smoke most unlikely...

08 September 2019 6,905 9 View

Aphid barcoding - will there be problems with plant-DNA when using the whole aphid?

Hello! I want to do a aphid-barcoding and was thinking of using the whole aphid and the standard primers for insects: LCO1490 and HCO2198. Now I wonder if I might get problems, if there's plant...

08 September 2019 160 2 View