Unlike Plasmodium falciparum, P. vixax clinical isolates could result from multi-clonal infections, because P. vivax relapses cause longstanding infections that can include sibling parasites inoculated by the same mosquito or unrelated parasites from separate mosquito bites, which makes lot of multiple allelic in SNPs dataset.

However, could P. falciparum clinical samples of high parasitaemia be subjected of multiplicity of infections (MOI)? In addition, is it important to assess MOI in P. falciparum samples (especially from areas where malaria is endemic) prior to whole genome sequencing?