Is MAO-B expressed in microglia?

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How to identify if a material is behaving like an anode or a cathode?

when we perform a cyclic voltammetry study, how can we conclude if a particular material is behaving like an anode or cathode with the help of that CV plot?

20 June 2024 1,453 3 View

Is it possible to get redox peaks in the negative potential window of CV plots?

The active material is a MXene/sodium based composite. All the active materials used as electrode material for SIBs shows redox peaks in the positive potential window but i have got in negative...

17 April 2024 7,024 0 View

Location Matrix For Damper?

Dear all, Can anybody help me to correctly write the Damper Location Matrix for a high rise building where dampers are provided at all floors. Thanks in advance From Bhargav

03 December 2023 2,310 2 View

Regarding Damper Location Matrix for a high rise building?

Dear sirs, I am working on high rise buildings. To determine the response (displacement, velocities, acceleration) State Space Method is used in MATLAB model. Can anybody help me to properly...

01 December 2023 9,719 2 View

Time history analysis for building-Scale factor?

Dear all, I am performing Time History(Linear) in ETABS using IS 1893-2016 along with static and Response Spectrum analysis. If the downloaded TH from PEER database is in the unit of G and I am...

14 July 2023 317 1 View

ETABS Fluid viscous damper?

Dear all I have modeled Viscous damper in ETABS using link but when I perform TH analysis and view the display cumulative response, the energy in VD remains zero. I may me making some mistakes in...

14 July 2023 9,499 0 View

76 Story benchmark building Problem MATLAB CODE?

Dear respected all I want to work on 76 story benchmark building problem. I want to know still the problem exist or not? Because I have tried to get problem MATLAB code from SSTL 2002 for third...

11 January 2023 1,713 1 View

Regarding 76 Story benchmark building problem?

Dear respected all I want to work on 76 story benchmark building problem. I want to know still the problem exist or not? Because I have tried to get problem MATLAB code from SSTL 2002 for third...

04 January 2023 1,720 1 View

76 Story benchmark building MATLAB CODE?

Dear all I am working on 76 story benchmark building with dampers. can anybody help me to download MATLAB code for 76 story benchmark buildings from SSTL 2000. I have tried 3rd generation code for...

02 January 2023 9,341 0 View

Regarding MATLAB code?

Dear all I am working on 76 story benchmark building with dampers. can anybody help me to download MATLAB code for 76 story benchmark buildings from SSTL 2000. I have tried 3rd generation code for...

02 January 2023 9,011 0 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Why not use CD11b staining to evaluate the purity of primary microglia?

I have noticed that, regardless of whether isolating primary microglia from humans or mice, purity is generally assessed using CD11c flow cytometry staining. Why not use CD11b instead?

02 July 2024 9,411 2 View

How to distinguish contaminated cells in a monoculture?

I am trying to grow microglia culture for 10 days and after day 2/3 there are certain flat non-microglial contaminating cells observed. They are rapidly growing and some are even growing on top or...

24 June 2024 5,325 2 View

Plz help me!!!!!! why not develop the phospho-JAK2 by LPS in Western Blot?

Hello, I am checking the inflammatory reaction that occurs when LPS is treated on BV2 microglia. When I treated LPS on BV2 cells, I confirmed a noticeable increase in IL-6 and I expected that JAK2...

12 June 2024 8,144 3 View

Can you suggest me an housekeeping gene for microglia?

I am working with microglia SIMA9 and I will perform a qPCR. If you have already worked with them, can you suggest me one?

05 June 2024 4,930 3 View

Is there any possibility for cultured mouse neurons more than 7 days?

Dear colleagues, I'm planning to start neuron mouse co-culture with astrocyte and microglia but I heard that it's difficult to keep it up. Any advices?

27 May 2024 4,905 3 View

What is the Estimated Protein concentration of 1 ml HMC3 cell lysate?

Hello everyone, I am currently growing Human microglia cells HMC3 in 100 mm plate. My question is what is the estimated Protein concentration (within range) of 1 ml HMC3 cell lysate? I usually...

25 April 2024 4,592 3 View

Difference in endogenous control expression and no sample left. Can the data be salvaged?

I have two groups that I collected microglia from and did RTqPCR. However, the CT values come up as extremely different (p

15 April 2024 6,268 0 View

Do you know the earliest activation time of microglia?

I am researching the microglia related neuroinflammation in CNS, and want to know the earliest time of microglia after acute inflammatory stimulation.

26 March 2024 6,209 1 View

Whether these primary microglia cells look fine in respective of their morphology?

I am currently learning to culture primary microglia cells from P0-P2-day-old pups, and after 14 days of culturing, I got these cells. I performed IBA1 staining to check purity, but still, I am...

12 February 2024 8,753 8 View