I used quercetin standard for TFC using AlCl3 and potassium acetate. While I was able to establish my calibration curve at 415nm, there was no color change for my sample. I even tried using 0.5mg/mL catechin in ethanol and the assay still did not work. The AlCl3 and potassium acetate were just 2 days old while catechin and quercetin were freshly prepared
Using catechin as a standard in the AlCl3-potassium acetate total flavonoid assay is not effective, as it does not form a detectable complex like quercetin does. Therefore, it's best to continue using quercetin or other suitable flavonoid standards for accurate total flavonoid content measurement.
Thank you for your reply. Unfortunately, my samples don't seem to have a lot of quercetin since they don't result to any color change upon interaction with AlCl3. I will try using ethanol dilutent but at least now I can try to quantify catechin using a different assay instead
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