I let you here one interesting reference:
Ultra-thin spin coated crosslinkable hydrogels for use in cell sheet recovery—synthesis, characterisation to application, M. E. Nash, W. M. Carroll, P. J. Foley, G. Maguire, C. O' Connell, A. V. Gorelov, S. Beloshapkin, Y. A. Rochev, Soft Matter, 2012,8, 3889-3899.
http://pubs.rsc.org/en/content/articlelanding/2012/sm/c2sm06466a#!divAbstract
Yes, it is possible, of course before polymerization, preferentially a UV - cross-linked polymer/Hydrogel.
Dear all;
the question should be clarified more regarding the fact that you are dealing with crosslinked or linear hydrogels. Also a precision should be given to the process used to prepare this this film because shear thinning under stress, temperature , and solvent is important to know for a precise answer. Regards
Thank you all. Actually I am going to crosslink the hydrogel after spin-coating. Besides, I cannot dilute the precursor solution.
Dear all,
TEM samples are usually prepared by cryo-microtoming, so actually I don't know why you are worrying and looking for a way to prepare thin film for TEM, or there is a problem when using microtoming. Please let me know. regards
Dear Aek Aziz,
You are right, I can use microtoming for TEM. But, the final product should be a thin film because of its application any way.
Dear Sahar Qavi;
would you please tell me how thin is this thin film you are intending to prepare, and is it transparent or opaque. Regards
Dear Sahar Qavi;
I think the following reference plus the attached files are in accordance with what you are looking for:
- Stimuli-responsive properties of N-isopropylacrylamide-based ultrathin hydrogel films prepared by photo-cross-linking. Langmuir. 2006 Jun 20;22(13):5911-5
Regards
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