I need to separate peaks of a carboxylic acid having a chiral center within it. I want to first separate the peaks of the 2 enantiomers in the racemic compound first in a suitable chiral column with a suitable flow rate and polarity. If it can be successfully performed, I will repeat the same for the chiral compound. I have heard that in many cases peaks of enantiomers of chiral carboxylic acids can't be separated.
- Yes, But too much difficult due to involvement of chiral columns. However, Enantiomers separate on the basis of three point interaction with the stationary phase.
what about chiral columns with basic functionality (amine) gives diastereomeric interaction would be able separate two diastereomers.
derivatization of carboxylic acid to bulky ester may lead later to separation on chiral column
What catalogues are you talking about M. Farooq Wahab ?Is it the sheets having info about the columns that are present in the column casing?
Separation of enantiomeric acids is indeed possible as Farooq points out. Success is largely determined by the structure of your acids. If the acid group is located on or very close to the stereogenic carbon and has aromatic and hydrophilic groups present as well, then a macrocyclic glycopeptide type phase, or Whelk-o or even the cinchona based phases could do the trick. I would research the macrocyclic glycopeptide phases first and can be found on Supelco‘s website (chirobiotic).
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