Is it possible to remove free drug from paclitaxel loaded protein coated nano suspension have the particle size of about 140nm?

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Jesus Valencia-Gallegos

Try dialysis.

Nisith Raval

You may dispense your naniparticles in such a solvent system, where your free drug may get dissolve whereas naniparticles will not... So upon centrifugation you will recover you naniparticles without free drug on its surface...

Right, just repeat that two or three times to get rid of the compound.

Mubashar Rehman

You can remove paclitaxel by ultracentrifugation. Wash pallet obtained with fresh solvent and repeat process until whole drug is separated.

You can also use dialysis membrane (or sac) of lower MWCO (