Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View

How should private health-care systems be improved to guarantee everyone's right to health?

COVID-19 pandemic has shown big social inequalities in countries with private health-care systems. Governments must pay greater attention to the right to health, that every human being must have,...

31 May 2020 7,357 23 View

I'm trying to set up a TR-FRET experiment in my lab but I'm having some troubles. Could you give some tips?

I'm working with an his-protein and I'm trying to obtain an IC50 between the protein and the biotinylated substrate and a known competitor. But the experiment is not working. I will add the...

20 April 2020 4,485 3 View

Problems with Google Scholar. Do you know how to fix it?

Dear All I am having some problems with google scholar. Some citations to my papers simply disappeared. I know that the number can be reduced when two different versions of a paper are...

01 July 2018 873 60 View

Is organic chemistry predictable?

It seems like organic chemistry consists of learning a bunch of reactions, but is there any qualitative set of rules for predicting the way a set of organic reagents will react? I know that...

30 May 2017 3,980 3 View

Are there any recognized / respected certificates for R programming or data analysis ?

Thinking about online and MOOC-type certificates for R programming and data analysis, are there any that are recognized and respected by potential graduate schools and employers? I guess if people...

11 March 2017 8,044 3 View

Hi all, what is the best method to determine the onset of bird migration and the end (day arrival), using GPS points?

There is a clear evidence in my data, but I would like to have also a statistical evidence

17 September 2016 7,140 4 View

Has anybody used Cas9 knockin mouse? If so do you know if every single cell express Cas9 constitutively?

Has anybody used Cas9 knockin mouse? If so do you know if every single cell express Cas9 constitutively?

29 September 2015 5,799 3 View

Is there a Cre line specific for the immune system?

22 June 2015 7,815 2 View

Is it possible to conduct AFM electrical measurements (such as EFM and KFM) in liquids?

I was wondering is any of the above techniques can be used for measurements in liquids. Thank you!

19 January 2015 3,178 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View