How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to study enzyme characteristics and activity for a recombinant bacterial laccase?

I am working on a novel bacterial laccase (recombinant), but I am not sure how to begin studying best conditions for enzyme activity. Studying optimal pH, optimal temperature,...

23 August 2020 4,791 4 View

Is it metol a possible substrate to check the activity of laccase in vitro?

Can I use metol ( 4-(methylamino)phenol sulfate ) as a substrate for the laccase activity? does an assay for this technique exist?

31 December 2019 1,913 1 View

I am preparing ABTS solution in Buffer 1 mM concentration of ABTS it appears in light green color. what colour it should have?

how can i prepare ABTS solution for laccase assay?

09 October 2019 3,933 1 View

How could I evaluate laccase enzyme produced by fungi qualitatively?

technical question

06 July 2019 8,863 8 View

Succesful large scale examples of biobased processes in paper or textile industry?

Are there experiences of bio based technologies as biopolish (textile industry), biopulping or biobleaching (paper industry). Hundreds of papers show promising results using fungal enzymes but it...

04 May 2019 2,550 2 View

Is commercial laccase from Novozymes [Novozym 51003] a typical blue laccase, despite its not visual blue solution color?

I am working with Novozyms 51003. It is a M. thermophila laccase expressed in Aspergillus sp. I collected the uv-vis spectrum for the commercial sample diluted in sodium acetate buffer. No 600 nm...

04 May 2019 2,648 5 View

Succesful large scale examples of biobased processes in paper or textile industry?

Are there experiences of bio based technologies as biopolish (textile industry), biopulping or biobleaching (paper industry). Hundreds of papers show promising results using fungal enzymes but it...

01 May 2019 7,479 2 View

What is the reason there is so much research on fungal laccases and only a few industrial suppliers?

What is the reason there is so much research on fungal laccases and only a few industrial suppliers? are they really used in the industry? or they have been replaced by something else? it seems...

05 February 2019 7,055 6 View

What's the difference between ABTS and syringaldazine?

01 February 2019 5,031 4 View

What is the reason there is so much research on fungal laccases and only a few industrial suppliers?

What is the reason there is so much research on fungal laccases and only a few industrial suppliers? are they really used in the industry? or they have been replaced by something else? it seems...

01 February 2019 4,236 7 View