Is it possible to prepare standard curves of guaiacol for laccase enzyme activity assay?
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After purifying Laccase enzyme (Bacterial origin), I tried performing enzyme assay using ABTS which is a known substrate. Still I am unable to validate its activity using the said substrate....
21 January 2024 5,159 0 View
I need to explain the mechanism of biotransformation of guaiacol in tetraguaiacol biocatalised by a plant peroxidase and I might know if the pathways of this mechanism differs or not differs from...
27 December 2023 2,810 0 View
I immobilized the laccase enzyme with glutaraldehyde on silica airgel support. The protein binds to the support, but its activity is quite low. The color change takes place very little in the...
06 September 2023 1,270 2 View
This for bacterial strains that i have isolated and i would also like to know which all dyes are better for other enzyme assays esterase, lipase, laccase, protease, amylase, manganese peroxidase....
10 June 2023 8,392 0 View
I want to check the activity of my immobilized laccase enzyme beads. How much beads you required to check the activity?
08 June 2023 1,262 2 View
1. I got red brown colony but not red brown clear zone. Can we say that it is a positive result 2. Tannic positive results can not be a positive on Guaiacol. Why? 3. Can I autoclave guaiacol and...
21 April 2023 2,028 2 View
I would like to optimize the immobilization of laccase on rice husk biochar. Prior the assay, I mix the rice husk biochar, glutaraldehyde and laccase in citrate phosphate buffer (pH 4.0) for 4...
10 January 2023 6,697 0 View
Looking for a physiological basis of the above mentioned events occurred in a grafted tomato plant.
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This is a paragraph from a paper: 'Laccase and peroxidase activities were quantified spectrophotometrically (Agilent, Cary 100 Bio) at 415 nm by detecting the oxidation of...
15 June 2022 661 5 View