Is vortexing safe to disrupt bacteria in suspensions for antigen preparations?
It is unlikely that you can uniformly disrupt bacteria by vortexing. You will have to sonicate with membrane disrupting agents [detergents/chaotropes etc] to get bacterial antigens in solution
What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...
06 August 2024 1,896 1 View
Effective collaboration between scientists from different disciplines is crucial for tackling complex, multifaceted research problems. what is the strategies and considerations that can help...
02 July 2024 6,367 5 View
Preprint servers play a valuable role in the scientific publishing workflow by accelerating the sharing of research, promoting openness and transparency, and diversifying the publication...
01 July 2024 4,022 2 View
The scientific publishing industry is facing several significant challenges today: Sustainability of the Subscription-Based Model Open Access and Funding Mandates: Peer Review Challenges Predatory...
01 July 2024 6,331 3 View
Effective public communication of research findings requires a combination of skills, including translating technical information, crafting engaging narratives, leveraging multimedia, and actively...
01 July 2024 1,330 2 View
I wan magazin within SCOPUS
22 June 2024 2,886 0 View
Hi, I’m master degree’s student at future university in Egypt and I’m looking for C. violaceum wild type (ATCC 12472) but I can't find it in my country. I want this strain to start my research...
25 February 2023 7,874 0 View
I am modelling eccentric braced frame with shear link, and I model the Link as multilinear plastic Links, but I need to know how to calculate the effective stiffness and damping the link is...
21 December 2022 1,097 0 View
I am make a model of eccentric braced frame and I need to run it with FNA but no Plastic Hinges Results appear
06 November 2022 7,685 1 View
what is the QC parameters in Analytical procedures of Plasma Atomic Emission Spectrometer .? In QC of the main parameter is the “accuracy” of the results. How the accuracy of the results were...
04 June 2021 7,672 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
02 August 2024 9,518 2 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
I am working on a project proposal (DFG project in Germany). I have the problem that I want to calculate how much funds I have to request for my test subjects (e.g., for qualitative interview of...
18 July 2024 1,690 0 View
I would like to perform a literature review at this time on augmented learning and learning augmented algorithms to enhance performance-guided surgery
06 July 2024 246 1 View
I am working on SCE and micronucleus assay. I completed around 30 samples of SCE and MN but suddenly the results get stopped and its been above 1 year struggling with the cell culture experiment....
02 July 2024 7,649 3 View
I need tips for isolating Ralstonia solanacearum from eucalyptus. I have used some methods, such as isolating from root exudates, but it didn't work. I also tried indirect isolation from macerated...
02 July 2024 9,807 3 View
The Evolution and Application of Spinal Anesthesia: Techniqu... What are the primary indications for choosing neuraxial anesthesia over general anesthesia in surgical procedures?
27 June 2024 5,763 1 View