i want to know the concentration of cream (extract as active ingredient) after the penetration test. i cant compare to quercetin because when i did the TLC, the extract doesn't show the same spot with the quercetin as the comparison. so, i can't make the standard curve from quercetin.
Check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6694887/
https://www.sciencedirect.com/science/article/pii/S1995764517304790
Also check https://www.sciencedirect.com/science/article/pii/S1658361216301469
https://www.intechopen.com/chapters/38925
Chinaza Godswill Awuchi Thank you for the references Mr. Chinaza👍🏻 But i have another question... After I read those journals listed, gallic acid used as the standard in the standard curve because it was for total phenolic/antioxidant content. But, what if I want to determine only the total flavonoid content in my extract? Because from the sources I read, the extract function is prioritized on the flavonoids... Thank you in advance for your help😊
Also check on this paper.
https://www.mdpi.com/books/pdfdownload/book/741
https://doi.org/10.1007/978-3-319-26811-8_8
https://doi.org/10.1038/s41598-020-60690-3
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