Bernice, you should get a copy of Standard Methods for the Examination of Water and Wastewater, published by APHA, AWWA, and WEP organisationsA. They have the methodology for measuring BOD documented very well. As Avinish stated above, the difference in the zero and 5 day reading is the BOD5, but you may need to dilute the water, or add a seed water, or do some other process to get a reading that is accurate. If you send me an e-mail (you can find my e-mail address on my web page at Purdue) I will also send you a lab write up on BODs and CODs.