How to perform correct peptide immunization in mice?

I would like to immunize mice with a pool of three MHC class I peptides, each having a lenght of 9-10 amino acids, in a total concentration of 100 ug. I will also use an adjuvant. Do I need...

16 July 2020 2,054 1 View

NTA for measurement of extracellular vesicles (EVs): How can different EV subpopulations in gradient fractions be measured and compared?

11 May 2016 7,027 1 View

Is there a method to purify Ig light chains, from Albumin?

Protein Purification Techniques: Hi, I tried to purify Ig light chains (LC) from MM or AL patients' urine with slight proteinuria, that means there is still albumin in there, messing up my...

14 October 2014 5,278 4 View

No title

03 March 2014 9,275 1 View

How did you solve the problem of getting parents` informed consent when conducting an online-study with children/adolescents?

We are planing an online study with german children and adolescents and are looking for soultions to make sure, that the parents agreed on the participation in the study (what is necessary when...

01 October 2013 4,036 1 View

Has anyone ever done an OVA specific long-term culture of murine OT1 T cells?

I am trying to establish a long-term culture of OT1 T cells using OVA. I've been trying to use T-cell depleted splenocytes and BM derived DCs as accessory cells. This works fine for the first...

10 March 2013 5,817 3 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View