Is it necessary to Water-Pik symbionts from coral tissues with seawater?

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Benedek PN, Sarjeant W. 1981..Nova Hedwigia. 35:313-356.?

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Nagalakshmi Radhakrishnan

coral lives only the seawater in tropical clear water with sunlight. so de-ionised water cannot use instead of seawater

Keshini Beetul

Thank you for your reply Nagalakshmi.

Sorry, I didn't include an important piece of information. What if the corals have been frozen?

According to Johannes & Wiebe (1970), de-ionise water may cytolyse the cell. Therefore, it is better to use seawater.