In case of In vivo experiment-
1) Is it mandatory to use same rat strain for both acute toxicity and anti-hypercholesterolemic activity?
2) Is it scientifically/ethically justifiable to use two different rats strain viz. Wistar rats (female) for acute toxicity and Sprague-Dawley rats (male) for anti-hypercholesterolemic activity for the same phytocompounds?
Using the same strain for both studies will make comparisons between activity and toxicity more meaningful, especially if your choice for activity studies relies on something unique about that strain (for example, unique expression of a receptor for certain phytochemicals).
However, using a standard strain for toxicity studies (for example, Sprague-Dawley) will better allow you to compare your results to published/historical data. If you have a strong rationale for doing it, using two strains can be acceptable.
If you are seeking to correlate the toxicity and anti-hypercholesterolemic studies and publish the results it is better to go with same strain. Though it won't make big differences practically but it will definitely draw the attention of reviewrs or readers.
Lee Koetzner Thanks a lot for the insightful clarification! Madhu C Lingaraju
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