Example code of calculating the Cramer-Rao Lower Bound (CRLB) for RSS positioning?

I am currently working on field of visible light positioning (VLP). Does anyone who can provide me an example code (Matlab or python) of calculating the Cramer-Rao Lower Bound (CRLB) for Received...

24 June 2019 7,315 2 View

How to remove trace amount of N2O from Helium?

08 February 2019 9,543 2 View

Does anyone who can provide me the Matlab code of Bootstrap algorithm for characterizing confidence interval?

I read some paper which utilize the Bootstrap algorithm involving sampling and resampling process to characterize confidence interval of measurement. Does anyone who can provide me the matlab...

08 February 2019 7,417 2 View

How to characterize the confidence interval of RSS measurements?

I am currently working on visible light positioning, one of the method is to use the received signal strength (RSS) based triangulation algorithm to calculate the receiver's location. My question...

05 February 2019 2,354 2 View

who has used Opal 7 immunology discovery kit from PerkinElmer?

has any one used Opal 7 immunology discovery kit from PerkinElmer to detect 6 protein biomarkers in paraffin section? I need advice for successfully using the kit

26 October 2017 7,248 3 View

Why can not my protein run into the gel during the BN PAGE?

29 March 2016 7,230 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View