I've been asked to help with a project determining genotypes of the gene coding for a specific enzyme in patient samples, but the most recent literature seems to only mention the use of qPCR with specific probes for the detection of the specific SNPs of interest. Due to the characteristics of the project, we can't do qPCR at the moment, just PCR, but we have access to sequencing as well, so I wanted to know if that's a feasible option, at least while we are able to perform qPCR

More specifically, not knowing much about the way sequencing is currently done beyond undergrad level lessons, I wanted to know if heterozygosity at a specific SNP could be picked up by commercial sequencing, i.e. getting two smaller, relatively equallly-sized peaks at a specific position instead of just a single peak, from a PCR-amplified fragment encompassing the SNP location. And if that's the case, how certain could I be that's actually a SNP and not just an artifact (assuming it's in the expected location)

Thanks in advance !!