It is known that phenol red mimics the action of some steroid hormones, particularly estrogen. What is the impact of using media with phenol red on the results of breast or ovarian cancer cells concerning cell growth, viability and proteins expression assays? has anyone noticed any difference in results when compared with media without phenol red?
I have used media with phenol red to culture MCF-7 cells, but the phenol red free media for tests for estrogen potency of chemicals. I think it is only needed to use the phenol red media for experiments there the purpose is to use the estrogen dependant response of the cell. In the culture of MCF-7 cells the media have to be added an estrogen for the cells to grow and here the phenol red has no effect.
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