Is it better to isolate rough endoplasmic reticulum first from virus infected plant to study viral replicase complexes?

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Prem Prakash Das

After infection, viral RNA facilitated with host factors to settled down on membrane of rough Endoplasmic Reticulum and form a punctate ER. Inside this punctate ER structure Viral RNA replicase complex remains present. So, How could I isolate this Punctate ER structure??

Xinghua Liu

Differential centrifugation，velocity sedimentation，or labelled by something and through flow cytometer?