There are several chlorophyll measurement systems using the principle described in Gitelson A. A., Buschmann C., Lichtenthaler H. K. (1999) “The Chlorophyll Fluorescence Ratio F735/F700 as an Accurate Measure of Chlorophyll Content in Plants” Remote Sens. Environ. 69:296-302

Dear Uma Maheswara Rao

this paper may help you

Article On the relationship between chlorophyll fluorescence quenchi...

(As it is written, your question looks a little odd!)

The reference to the work of Lichtenthaler et al., cited by others, is indeed a must. So, I will just give side remarks.

In vivo, chlorophyll fluorescence varies with the functioning of the photosynthetic apparatus, so the best would be to block it at the highest level with DCMU to normalize the results. This can be done on a separate aliquot of the bulk ocean water.

In vitro, a strict relationship between chlorophyll concentration and fluorescence requires diluted solutions to avoid self-quenching.

(As it is written, your question looks a little odd! What do you mean by "energy from of chlorophyll fluorescence"?)

The reference to the work of Lichtenthaler et al., cited by others, is indeed a must. So, I will just give two additional remarks.

In vivo, chlorophyll florescence varies with the functioning of the photosynthetic apparatus, so the best would be to block it at the highest level with DCMU to normalize the results. This can be done on a separate aliquot of the bulk ocean water.

In vitro, a strict relationship between chlorophyll concentration and fluorescence requires diluted solutions to avoid self-quenching.