Yes, this is possible, but you should ask the column manufacturer if it is possible to use another solvent in this column. If the column is not supported, it is possible to damage the column. Still, there is another problem! you should also calibrate the GPC with chloroform solvent!

I don’t recommend this - there is a good chance your polymer will precipitate in the system - if you are lucky it just mucks up the injection part for you to clean , if not you lose the column.

when you say only partially dissolves - is this low

solubiity (so use samples below the solubility limit) or fractionation - high MW not soluble (which means the GPC will be of a different Material than the solid).

Dear James E Hanson

Thank you very much for your answer! Basically my polymer is PHB (Polyhydroxybutyrate) which I belive is not soluble in THF. However, I found this paper in which they did dissolve the polymer in Chloroform and used it on a THF-eluent SEC. I did run a few samples already and it seems to me that everything is alright!

Article Controlled degradation of polyhydroxybutyrate via alcoholysi...