Dear Dr Mohamedm thank you for your interest,

A neonatal kid displayed a congenital, spherical, subcutaneous mass at the lateral side of the neck. Histopathology showed a highly cellular mass consisting of tumor cells at the myotube stage of muscle development. The tumor cell were mostly tube shaped, the cytoplasm was lucid; the nuclei were elongated and centrally aligned within the tubes. These tumor cells were arranged in parallel as bundles of fibers. Now, I need a confirmation of my first suggestion using the Immunohistochemistry. The question, could I use the commercial AB of human origin for RMS or should use specific one for goats? 

Thank you Dr David,

Under microscope it is a case of RMS, but for publication, I thing IHC is necessary.

Hi Ahmed,

Myosin IHC might be helpful, however be aware that majority of the secondary antibodies are raised in goat, so there is a possibility for high background staining.

Combination of PAS-Levanol Fast Cyanine staining might help you as well.

Best,

Ivan

Thank you very much Dr Ivan for your important information, I may return for you again when I start with the protocol.

Ahmed