is hydrogen gas in a saturated hydrogen rich water solution in a tightly-closed flask like those in photo attached down is stable at high temp ( specifically 42 degree celesius or it will release in some way ?
no dear Rick Manner . i actually ask about Molecular H2 gas when bubbled into water resulting saturated hydrogen rich water mixture. is it stable at a temperature higher than room temp ( specifically 42 degree " heat shock degree").
Thank you for clarifying your question. Yes H2 is stable at 42 C if you bubble it through water. If CO2 is present in the water the water gas shift reaction H2 + CO2 H2O + CO may occur. H2 is a strong reducing agent so it will react with any oxidizing agent that might be present and it is small enough to diffuse through just about any material over time. But it will not react with H2O at 42C because H2O is already reduced.
thanks dear Rick Manner for your reply. kindly please i need to use H2 for alleviation of heat stress on a microorganisms as H2 works as a very good antioxidant. but i will use hydrogen bubbled in water ( Hydrogen rich water) in a shaking culture at 150 rpm in a plugged flask. So, Do you think skaking may cause hydrogen gas release through those plugs or to the air trapped in the empty space inside the flasks?
H2 is able to pass through almost any container you try to hold it in. The question is really how much will escape not if it will escape. I am not an expert on this type of experiment but I do not believe the amount of H2 lost will be significant but you will probably lose some.