Is His-Tag by itself immunogenic?

01 November 2023 1 3K Report

Do we have an evidence that His-Tag related impurities are non-immunogenic? Also, does H6 or H10 get cleaved in the blood stream by exo-peptidases? I want to know the permissible limit of the His-Tag related impurity in the bio pharmaceuticals.

Adam B Shapiro

Please see this previous thread

https://www.researchgate.net/post/Is_6xHis_tag_compatible_with_therapeutic_GMP_protein_production

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