Do we have an evidence that His-Tag related impurities are non-immunogenic? Also, does H6 or H10 get cleaved in the blood stream by exo-peptidases? I want to know the permissible limit of the His-Tag related impurity in the bio pharmaceuticals.
Please see this previous thread
https://www.researchgate.net/post/Is_6xHis_tag_compatible_with_therapeutic_GMP_protein_production
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