Hello everyone,
My lab plans to use Phospholipid Fatty Acid Stable Isotope Probing (PLFA-SIP) to see if a specific microbe utilises a certain substrate as a carbon source. Luckily our microbe has a unique lipid molecule only found within its group.
However, we are trying to figure out what analytical machine will be best for our purposes. I have found in some literature using gas chromatography/isotope ratio mass spectrometry (GC-c-IRMS). Therefore, would this be suitable for detecting potential isotope uptake in our microbe?
Thank you very much
Calvin Cornell
Hello Dr. Noel Davies,
Fantastic! Thank you very much for telling me this and for your help it is much appreciated!
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