Attempting to isolate iron reducers from various sediments, unsure as to whether FeCl2 should be added to ensure anaerobic enough conditions - not clear whether this is commonly practiced from papers.
Yes....it required as a precursor for the reducing microbial species for building the deficient proton environment
I always used FeCl2 as mild reductant during cultivation of iron reducers and got good result.
What concentration do you guys always use?
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