In some cases you must need to do derivatization for GC-MS analysis. Although it is better to do it can enhance your results in a positive way.
Good luck
It is not a requisite step but maybe it will enhance the chromatography of polar substances in a crucial way. Alkaloids, essential oils, etc...
Dear Asit Ray,
Derivatization is not a requisite step for phytoconstituent analysis of plant extracts by GC-MS. if you are working with non polar extracts than there is no need of derivatization of plant extracts as such. But if you r dealing with polar extracts which contained polyphenolics, flavonoids etc. which are not volatile in nature in this case you have to derivatized the extract first prior to GC-MS analysis in order to make these compounds volatile. Simply in GCMS analysis the compounds which are not volatile in natureshould be derivatized prior to analysis in order to make them volatile. read the file attached with for more detail.
With Regards
Hi,
You make derivative for long chain fatty acids that have the tendency to stick to the GC column and clog it. They can be converted into esters using acid chlorides
Derivatization can also be useful in measuring phytohormones (and other metabolites) via GC-MS. This paper details a powerful method to simultaneously measure many such compounds:
https://www.researchgate.net/publication/8395171_The_use_of_vapor_phase_extraction_in_metabolic_profiling_of_phytohormones_and_other_metabolites
Article The use of vapor phase extraction in metabolic profiling of ...
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