I'm staining a polyacrylamide gel with Coomassie Blu Brilliant R250 (Merk 12553) dissolved in 10% acetic acid and 40% methanol. Is this dye solution compatible with future analysis of mass spectrometry?
You should be fine. you can always cut your bands out and destain them until they are clear again if you're worried about it. Keratin is what kills mass spec every time so cover up your skin and hair...yes the beard too. haha
Hi Nicola,
It is compatible in the sense that you can easily destain the gel before mass spectrometry. If you do not destain the gel, you will end up contaminating your HPLC column with Coomassie Blue. The dye competes for binding of your peptides generated from your protein, to the analytical or guard column.
So, the answer is yes, it is compatible, as long as you completely remove it before mass spectrometry analysis.
Best,
Hediye.
Keep all such reagents away from the GC and LCMS. Make sure it al washed out of the gel before you extract with ACN/H2O.
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