How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

Dear professors, i am confused with how to figure out the tea bag index?

Hello, i am confused with how to figure out the tea bag index,could you please tell me how to compute the letter "a" and "k". Can i use R or Sigmaplot to fix it?

09 December 2020 6,705 3 View

Are there any easy way to test the activity of CYP51?

I'm looking for a relative easy way to test the activity of CYP51. Are there any way that I can measure it without using LC-MS or gas chromatography. A simple absorbance or fluorescence test will...

08 October 2020 6,687 3 View

Strain Hardening Phenonmenon under Uniaxial Tension when using ABAQUS CDP?

Dear all, I encountered some problems when using the ABAQUS CDP model. Could you please give me some suggestions? I did a monotonic uniaxial test using a single 3D solid element (C3D8R) and the...

04 October 2020 7,111 3 View

How to extract RNA from pancreas?

RNA extraction from pancreas of ruminants is very difficult due to the existence of abundant RNase. Previously, our research group tried to remove the pancreas from animal immediatley after...

20 September 2020 1,097 2 View

How to get bending moment in Ansys shell181 element?

Hello, I would like to compare the bending moment of a tube with beam and shell element in ansys? The tube has the same dimension in beam and shell element. In beam element, i can get the...

24 August 2020 2,352 1 View

Buckling Analysis - Risk vs Ex/Implicit vs Static Stabilised - Displacement vs Force Control ?

Dear all, I am studying how to conduct lateral-torsional buckling analysis of a simply supported beam. The beam is purely elastic, and the boundary conditions are shown below. I started with a...

21 July 2020 3,761 2 View

How do I detect the onset of each period of a noisy periodic signal (MATLAB preferred)?

I have a noisy periodic signal, like the attached picture, is there a way to extract the time of each onset of each period (shown by red arrows)? MATLAB preferred other programming languages also...

16 July 2020 5,988 8 View

Roche has stopped its Universal Probe primer design service, does anyone know where or how we can design primers to fit Universal Probe Library?

Roche announced the Universal Probe primer design service will be stop in the end of 2020, but the primer design website has already been shutted...

10 February 2020 4,745 2 View

Reusing C18 ziptip while desalting the peptide sample before LC-MS/MS ?

Reusing C18 ziptip while desalting the peptide sample before LC-MS/MS ? Hello everyone I have a question while using ziptip Do you suggest that reuse the zip after one sample binding and...

11 December 2019 7,865 3 View

Is there any pipeline for 16s metagenome bacterial classification based on their functions?

In 16s rDNA metagenome analysis, after sequencing data. I want to classify bacteria based on their functions. I am looking for a Pipeline or any software that can do this job?

13 January 2021 533 6 View

What the best way to improve Illumina genomic assembly with nanopore metagenomic data?

I have the Illumina reads and assembly of my bacteria from pure culture DNA sequencing and the nanopore sequencing data from the metagenome (DNA of co-culture). Does anyone know the best way to...

14 July 2020 6,516 3 View

Any recomendations for selective lysis of Eukaryotic cells stored in RNAlater or Ethanol?

Hello everybody, --just a brief intro about the background of my question-- I have to analyse the gut microbiome of a marine fish through whole metagenome sequencing. Going through literature, I...

18 February 2020 8,460 1 View

Total DNA Quality for 16S Amplicon Sequencing ?

Dear all, I performed DNA extraction using Qiagen DNeasy Blood and Tissue Kit, followed the protocol with an addition of 2ul RNase A with incubation of 2 min at R.T after adding proteinase K....

01 February 2020 5,395 4 View

Does genomic DNA bands smear on gel are degraded DNA?

I have to send my sample for metagenomics sequencing but my genomic DNA shows up a clear band with thin and long smear. It looks like my sample has degraded but I'm not sure. I read somewhere that...

15 December 2019 206 3 View

How to perform differential gene expression analysis for a DESeq2 dataset with multiple factor levels?

I'm a novice to RNA-seq analysis and DGE, hoping to get some good ideas moving forward. For context: I have 15 samples, 3 replicates each of 5 sample types. They're mRNA samples obtained from...

09 October 2019 8,206 4 View

How to extract good quality total DNA from insect larvae for metagenomic purpose?

Good day, currently I am practicing extracting total DNA using drosophila melanogaster (fruit fly) adult as well as larvae as my sample with Qiagen DNeasy Blood and Tissue Kit. However, I keep...

28 August 2019 601 3 View

How to extract good quality total DNA from insect larvae for metagenomic purpose?

Good day, currently I am practicing extracting total DNA using drosophila melanogaster (fruit fly) adult as well as larvae as my sample with Qiagen DNeasy Blood and Tissue Kit. However, I keep...

07 August 2019 5,060 3 View

Metagenomic analysis of bacteria - where to start?

I have no knowledge about metagenomic analysis. Could you suggest me an article or a website to start with from scratch? Please suggest FROM YOUR PERSONAL EXPERIENCE! Thanks!

06 July 2019 3,555 10 View

How to do alignment of binned genomes for diagnostic qPCR?

Hi, I've generated three binned genomes from soil metagenome samples. These binned genomes are collections of contigs, and I would like to find a program that could efficiently perform an...

06 July 2019 7,495 2 View