How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What properties of water shall be analyzed and evaluated to test the effectiveness of activated carbon?

I would like to ask what other properties of water (pH and such) shall be analyzed and evaluated to test the effectiveness of activated carbon from filtering the contaminants present in the water?

20 February 2021 9,422 8 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

What is the chemical composition of chlorellin?

The chlorellin is the chemical compound which is first extracted from Chlorella vulgaris by Pratt in 1944. Spoehr in 1949 wrote that it is a mixture between fatty-acids and hyrdocarbons and its...

25 January 2021 8,538 1 View

What Contaminates Cause Loss of Resolution in Plasmid Sanger DNA sequencing?

We use an AB 3130xl Genetic Analyzer for Sanger Sequencing, and on occasion, we see our plasmid samples struggle with wavy, loss of resolution (as seen in the attached image). Our general...

14 January 2021 7,346 6 View

Can anyone put up some light on how added urea could be detected from food item(not milk)?

We doubt that some people add urea in fish based food item, any one can suggest some method by which we can detect the uear qualitative and quantitativly.

16 November 2020 6,839 3 View

Pollutant extraction from contaminated soils for toxicity assays using human cell lines. How to resuspend the contaminant crude extract?

To study the toxicity of a contaminated soil with petroleum and heavy metals using human cell lines as model organism, we need to perform a pollutant extraction step before carrying out the...

08 November 2020 1,421 4 View

Is there any advantage on adsorption process to be done at solution pH near to 7 (neutral)?

Generally, it has been reported that some of the adsorption phenomena are of pH dependent. Some studies have shown that the effective removal of contaminants from wastewaters/aqueous media can be...

04 November 2020 8,852 5 View

Which parameter can be used to best compare adsorption capacities of different adsorbents?

If I were to compare the adsorption of a given type of contaminant on different types of adsorbents, which parameter would best enable the best comparison? The adsorbates can be either organic or...

14 September 2020 3,592 4 View

How can I differentiate between cyanobacteria and microalgae in agar plates?

I'm trying to isolate cyanobacteria from fresh water and soil. I've plated several dilutions of my samples into BG11-agar plates and I have a mixture of green colonies/halos and brown stains which...

06 September 2020 8,959 4 View

Alternative to use of ethanol in extraction process?

Dear all., I am using ethanol as part of the extraction process of Chlorella vulgaris carbohydrates. Since I am trying to scale up a bit the whole process, I should use much more ethanol, that in...

27 August 2020 1,875 5 View