Trying to make up some different media to isolate soil actinomycetes, and also ocean actinomycetes. I was going to go with actinomycetes isolation agar in addition to a few others, but was wondering how important the asparagine really is.
Sodium Caseinate----2g
Asparagine---0.1g
Sodium Propionate----4g
Then some trace metal salts, and sometimes addition of glycerol.
Was also looking at Soya Flour Mannitol media, Triptic Soy Agar, and Starch Casein Agar, with perhaps addition of autoclaved ocean water when isolating from saline mud samples.
Suspend 22 grams of the medium in one liter of distilled water. Mix well. Heat with frequent agitation and boil during one minute or until completely dissolved. Add 5 grams of Glycerol and mix well. Distribute in appropriate containers. Sterilize in autoclave at 121ºC for 15 minutes.Asparagine nitrate agar medium is prepared for isolation .Starch casein and asparagine agar are used for isolation of soil actinomycetes.
Thanks for the responses. The humic acid vitamin agar seems pretty interesting. I'm trying to follow in the footsteps of Albert Schatz and discover something great in a basement! www.basementbiotech.org is my blog where I write about some things.
The hard part though is the structure elucidation and bio-assay guided fractionation of extracts. Since so many things have been discovered and re-discovered, I figured looking in the ocean's soil might provide a higher chance of finding something novel.
www.basementbiotech.org
- Badges
- Science topic
- Similar topics
- Agricultural Science
- Soil Science
- Soil Analysis