How to obtain Contig sequence from BAM file?

I have use HISAT2 for alignment . Now i want to obtain contig sequence from from BAM file in galaxy server? I have used Samtools View. That is giving me some sequence but I am not sure whether it...

12 February 2021 3,262 4 View

Could you please share your experience in learning SPSS?

I would like to learn SPSS. Could you please guide me or share your experience if you feel it would help me to learn SPSS? Thank you very much indeed!

08 November 2020 7,357 6 View

How do I compare time domain solution with frequency domain?

I am solving EM wave scattering by PEC cylinder numerically and wanted to compare the scattering width. My result is in the time domain which is evolving with time but the analytical solution is...

11 October 2020 770 3 View

Is it possible to consider intermittency of renewable energy sources by using normally (Gaussian) distributed random numbers ?

Consider wind speed for wind power and solar radiance for solar power. Is it possible to consider intermittency of mentioned sources using normally ( Gaussian ) distributed random numbers in which...

29 January 2020 5,731 3 View

Could we download LANDSAT LST images from earth explorer site corrected atmospherically or the products corrected proposed are just for optical bands?

Could we download LANDSAT LST images from earth explorer site corrected atmospherically or the products corrected proposed are just for optical bands?

12 January 2020 9,036 3 View

How to prepare 1U of lyophilized powder of Bacillus stearothermophilus?

First of all, i have narrow knowledge about antidiabetic assay due to this is my first enzymatic test and i have no one to refer. I have a method but when i tried to google every each of the...

05 January 2020 4,629 3 View

How to compute unknown parameters in an equation from experimental data?

Is there a way to compute some unknown parameters in an equation using curve fitting using experimental data? I am upload a picture which contains the function and three unknown parameters and...

25 December 2019 2,184 7 View

From the Table attached, why Retentivity is decreased for Bi:ZnO 3% and for Bi:ZnO 5%, Coercivity is decreased but Ms is increasing linearly for all ?

Hi all, referring to the table attached, it is seen that when ZnO NWs are doped with Bi at different concentrations, there is an increase in magnetization values initially. But we see by doping...

08 October 2019 3,938 3 View

How to make 500 unit/ml catalase solution using catalase protein from Sigma?

13 August 2019 7,931 3 View

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10 March 2019 8,910 11 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View