Is aromatisation the only source of estrogen in adult male?

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Hey, does anyone know any effective way to separate virus from exosomes fraction? my problem with the separation of the EVs in this system is the fact that the virus that we work with particles...

06 December 2020 4,765 2 View

Number of cells for extacellular vesicles extraction?

hello! I want to extract vesicles from MDA MB 231 cell ligne, culture in serum free media for 48h, would you help me with th number of cells required to have the vesicles? thank you

03 December 2020 6,488 2 View

What are the XRD analysis conditions of the cement, sand and Limestone ?

I am a Phd student and I need to do XRD analysis on my pouder samples namely ( cement- sand- limestone and calcined clay). I was asked asked to specify the analysis conditions: -the nature of...

07 October 2020 5,737 6 View

Can anyone recommend me some articles about Fire endurance of wood concrete?

Are there documentations about Fire endurance of wood or wood shavings concrete? The literature is not very clear about this .

12 August 2020 8,203 3 View

Is it possible to use plant extract as natural sterilizer?

For economic reasons, sterilzation against covid_19 is very necessary, so is it possible to use plant extract?

11 May 2020 2,674 14 View

Which statistical analysis is more appropriate for my study?

I am trying to find the relationship between the use of defence mechanisms in early adulthood as predictors of neuroticism. Those who use more defence mechanisms will score higher on the...

14 April 2020 3,353 7 View

How do I carry out performance evaluation on a sorting machine?

I design a prototype for sorting tomatoes based on size and colours. The prototype will sort big red, big green, small green and small red

12 March 2020 2,386 3 View

Preparation of the mix for Standard PCR (without using a kit)?

Hi, Please anyone already prepared the mix for Standard PCR? But without using a commercial kit. It is for staphylococcal enterotoxins genes. I am looking for the concentrations of each...

08 February 2020 4,487 6 View

How to deal with mental health issues?

I feel communication helps in coping such issues to some level.

16 August 2019 7,405 31 View

Course -Preparation advices !?

Dear colleagues, As I am new in the domain of teaching courses, so it takes too much of time to prepare my course, saying for example: cryptography course, web development,..etc,... So, my...

30 May 2019 6,236 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

How can I interpret a ratio variable?

Dear colleagues, I am running a regression in which I have the following regression FoodExp = 67.04 DepRatio +.....e My interest is in the interpretation of the coefficient on the dependency...

16 February 2021 2,058 2 View