I need help with developing rocket immunoelectophoresis.  I am using 1% low EEO Agarose cooled to 55C in TBE buffer.  I am adding 100 mg/mL antigen diluted in TBE buffer at 2% of the agarose volume.  I run samples of various concentrations diluted in TBE buffer at 100V in a native running dye.  I am not getting any precipitation fronts after 24hrs at 37C in a moisture box.  Any ideas as to why?