What are the possible reasons for a primer dimer to occur in a PCR, LAMP reaction?

I am currently working in an isothermal amplification technique called PSR ( Polymerase spiral Reaction). I have followed the reaction mixture from previous articles but i am getting primer dimers...

15 February 2020 2,826 3 View

HL60 cell differentiation using 1.3% DMSO?

07 October 2019 6,851 2 View

What are the effects of flour gel hydration properties on bread crumb structure development?

Dear Colleagues, Can anyone describe how Water Absorption Index, Water Solubility Index and Swelling Power of the flour type we use for bakery products can affect the porous crumb structure...

08 June 2019 4,591 2 View

Issue with PCR amplification of an amplicon ?

I have done a 16S rDNA amplification of a metagenomic sample. I got a clear band near 1.5kb in the gel. Later on purification and again when we do the same PCR with the exact same primers and...

25 February 2019 4,532 5 View

Can any one give the optimum conditions to prepare Cdo-Zno thin film by spin coting technique?

can any one give the optimum conditions to prepare Cdo-Zno thin film by spin coating technique?

19 February 2019 1,979 2 View

Why is the fungal culture not growing in the agar medium but grows in broth ?

Trichophyton rubrum and Microsporum canis are the 2 fungal cultures that i need to assay ( well diffusion assay), but i have encountered a problem, the fungal culture is not growing in the plate....

23 December 2018 2,632 6 View

Is there any alternative method for OLS regression?

I have cross-sectional 144 firm level observations in order to measure the stock performance. OLS model (multiple regression) results are free from autocorrelation and heteroscedasticity errors....

18 December 2018 7,460 20 View

Measuring long run stock returns using Cumulative Average returns (CAR) and Buy and Hold Average Returns (BHAR). Can ultimate answers be same?

16 December 2018 1,608 3 View

Can TITANIUM butoxide monomer chemicals are useful to prepare titania nanoparticles?

is titania nanoparticle is prepared from titanium butoxide monomer?

29 January 2018 9,567 3 View

How to prevent DNA binding protein precipitation while concentating?

I have DNA binding protein and I am getting a conc, of 50um from 50ml BL21 DE3 culture (I will be scaling this up to 1mM). The protein is Hexa-His tagged at C-terminus and to elute the protein...

23 August 2017 9,167 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View