Excel formula for assigning grade?

I have a excel sheet with positive and negative value. I have 4 grades to be assigned. How can I use a formula to assign the grades? I have tried using the IF nested function but due to negative...

21 October 2020 5,648 7 View

Statistical test for Inter and intra rater agreement between 2 observers for gradings? any excel based template for the same?

Hello, I wish to calculate inter and intra rater agreement between 2 observers who are gradings Xrays for degree of bony fusion into 6 grades. In all there are 1750 xrays. Which statistical test...

11 August 2020 1,001 4 View

How common is COVID19 among pediatric population?

Most papers not giving in details about the disease in children. Any one have idea about it. Is Children are protected?

09 March 2020 6,642 39 View

Can a use of Demineralized water with PH of 5 to 6 to clean aluminum cause corrosion?

06 March 2020 793 6 View

Which is the best ELISA assay kit for the measurement of mouse serum and urine creatinine?

Hi, I am looking mouse ELISA kit for the evaluation of creatinine level in serum and urine. Could you please suggest me which kit is most reliable for this assay? Regards, Nirmal

03 December 2019 824 4 View

How many maximum number of authors should be included in a good quality review paper?

During the writing of a review paper it is very difficult task to add number of authors. However it varies person to person and their contribution in paper. but, there shld be any ideal number...

01 August 2019 3,687 16 View

Why high alumina cement is resistant to sulfate?

In OPC, C3A and C2AF has high affinity to sulfate (gypsum) to form mono-sulfate or ettrengite. But in contrast, high alumina cement is good at resisting when exposed to SO4- environment. Why?

05 June 2019 2,994 8 View

Hi everybody I want publish my Ph.D work Can anybody suggest me some publishers who publish thesis work ?

18 September 2017 9,747 11 View

What is good protocol for fixation of rat Kidney and eye balls?

Does anybody know good protocol for rat kidney and eyeball fixation? I have frozen kidney and eyeballs in -80 and want to fix them for IHC. I tried few protocols but eyeball get broken or...

20 April 2017 6,004 3 View

Can we isolate gDNA from -80 stored brain homogenates (no tissues)?

I have brain homogenates (supernatant after centrifugation) stored in -80 long ago. I need to do genotyping in those samples but i do not have blood of those samples. What i have is homogenates...

13 September 2016 2,797 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Is the sinovac vaccine safe and effective for people over the age of 60 ?

Dear respective researchers, I was asked by my institution to be vaccinated this week using the sinovac vaccine. However, from some of the information I got, this vaccine is not recommended for...

01 March 2021 7,781 6 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View