Rotavirus is an important causative agent of AGE in children lessthan 2years. To prevent mortality and morbidity it needs to be contained beside vaccination.
I F OLLOW THE QUESTION
Who are the scholars who work at the intersection of religion studies and the built environment?
21 April 2024 1,195 3 View
I have built my own SPR setup and I wanted to test the overall design. It essentially contains all the necessary SPR components like the laser, glass with gold coating, and a detector to capture...
10 March 2022 187 0 View
One of my PhD Scholars wants to apply the Torrance Test of Creativity for assessing the creativity of secondary school students in West Bengal for his research. So kindly suggest an ethical way...
27 December 2021 1,185 4 View
Many of the research papers I looked through have the laser or light source at one end of the fiber optic cable and a detector at the other end. But I wanted to know if there was any research on...
18 December 2021 8,354 14 View
I came across a Fibre Optic SPR sensor used with a phone and I was trying to figure out what thickness they used for their sensing...
13 December 2021 3,343 3 View
I see small aggregation-like particles in primary neuron culture from the hippocampi of rats. Can anyone suggest what are these ? And can we go with drug or other treatment assays with these...
08 September 2021 224 3 View
The SPR slide is made of glass with a 50nm Au layer on top. Above this layer I can attach the streptavidin with one of the following procedures I found on another post for a bare glass slides: 1)...
05 April 2021 7,331 1 View
Surface plasmon resonance (SPR) slides contain a glass slide with 50nm thick gold coating on top of it. Instead of using the Kretschmann setup with a bulky prism and having to worry about...
03 April 2021 8,739 5 View
Typical SPR slides are made of glass with the 50nm gold coating on top. But I wanted to know how I could create SPR slides made from clear flexible plastic with the coating on top so that I can...
03 April 2021 3,524 2 View
I have a excel sheet with positive and negative value. I have 4 grades to be assigned. How can I use a formula to assign the grades? I have tried using the IF nested function but due to negative...
21 October 2020 5,818 7 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View
I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...
16 July 2024 4,604 0 View
I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...
12 July 2024 7,145 2 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View